Enraizamento in vitro de mogno (Swietenia macrophylla King)

Abstract

O presente trabalho teve como objetivo comparar diferentes concentrações de AIB e ANA para indução do enraizamento de ápices e brotações de mogno (Swietenia macrophylla King) in vitro. Os explantes utilizados foram obtidos de experimentos de multiplicação in vitro com, no mínimo, 2cm de comprimento, com suas bases cortadas de forma transversal. O meio utilizado foi o MS (Murashige & Skoog, 1962), porém adicionado de vitamina do WPM (Mccown & Lloyd, 1981), suplementado com 3% de sacarose, ágar 0,7% e PVP (Polivinilpirrolidona) 0,1%. Foram testados AIB a (0,1; 1,0; 3,0 e 5,0 mgL-¹) e ANA a (0,1; 2,0 e 5,0 mgL-¹). Os explantes permaneceram por cinco dias nos meios de cultura, em contato com estas auxinas. Após, foram transferidos para meios novos sem os reguladores de crescimento e com os sais do meio MS reduzidos à metade. A auxina ANA na concentração de 2,0 e 5,0 mgL-¹ foi mais eficiente para indução do enraizamento, com percentual acima de 70%, tanto em ápice quanto em brotações.

This research objectived to compare different concentrations of IBA and NAA for induction of the rooting of apical segments and shoots of mahogany in vitro. The used explants were obtained from experiments of multiplication in vitro with at least 2cm of length, with cut bases in a traverse way. The substrate used was the MS (Murashige & Skoog, 1962), it was added vitamin of WPM (Mccown & Lloyd, 1981), supplemented with 3% of sucrose, agar 0,7% and PVP (Polyvinilpirrolidone) 0,1%. IBA were tested the (0,1; 1,0; 3,0 e 5,0 mgL-¹) and NAA the (0,1; 2,0 and 5,0 mgL-¹). The explants remained for five days in the culture medium, in contact with these auxins, after they were transferred to new substrate without growth regulators and with the salts of the MS substrate reduced in half. The NAA in the concentration of 2,0 and 5,0 mgL-¹ was more efficient for induction of rooting, with percentage above 70%, both apical segment and in shoots.